1. Consider the following statements.
I. HPLC is a qualitative process.
II. HPLC is a quantitative process.
III. Triplets give information about molecule structure.
Which of the above statements apply to high-performance liquid chromatography (HPLC)?
A. I only
B. II only
C. I and II only
D. I, II and III .

Solution

2. The graph below shows the HPLC calibration curve for folic acid.

A 1.00 mL sample containing an unknown concentration of folic acid is diluted to 1.00 L. The diluted sample is analysed using the calibrated equipment at the same conditions. The folic acid peak area is found to be 0.35 What is the approximate folic acid concentration in the 1 mL sample?
A. 18 pg mL⁻¹
B. 18 ng mL⁻¹
C. 18 µg mL⁻¹
D. 18 mg mL⁻¹

Solution